Conference publications

Polyelectrolyte Protein Complex. Polyallylamin Influence on Structure and Functions of Urease

Durdenko E. V., Dybovskaya Yu. N., Tichonenko S. A., Saburova E. A.

"Математика. Компьютер. Образование". Cб. трудов XVI международной конференции. Под общей редакцией Г.Ю. Ризниченко Ижевск: Научно-издательский центр "Регулярная и хаотическая динамика", 2009. Vol. 2. Pp. 145-157. (accepted)

Influence of polyelectrolyte polyallylamine (PAH) on structure and catalytic characteristics of urease (Canavalia ensiformis) by methods steady-state kinetics, fluorescence spectroscopy and circular dichroism is studied. It is shown that this polyelectrolyte is strong inhibitor of urease (50 % inhibition of enzyme with concentration PAH of 0,5 mkg/ml at neutral values рН). It is assumed regulatory role specific cation-binding sites on a molecule urease, defining structural dynamics a complex enzyme-polyelectrolyte.

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