Organization of bilayers at multilamellar liposomes, formed from synthetic phospholipid of from mix of natural phospholipids, under the actions of phenoksan and melafen

Alekseeva O.M., Krementsova A.V., Krivandin A.V., Shatalova O.V.

N.M. Emanuel Institute of Biochemical Physics of Russian Academy of Sciences, Russia, 119334, Moscow, Kosygin St. 4, +74959397409,

For investigation of biologically active substances melafen and phenoksan effects the consistent number of experimental objects was used two models – multilamellar phospholipid liposomes, formed from synthetic individual phospholipid dimyristoylphosphatidylcholine (DMPC), and from mix of natural phospholipids of egg lecithin. The registration of experimental objects reactions to the actions of BAS was held by the methods: DSC, small-angle x-ray light scattering (SAXS). The concentration regions of BAS have been determined by DSC under the application of phenoksan (10-5 M) and melafen (10-16 M - 10-3 M). These regions were without the destruction of microdomain organization at bilayers of multilamellar phospholipid liposomes, formed from DMPC. Melafen over a wide range of concentrations didn’t disrupt the structure of such multilamellar phospholipid liposomes, but caused the changes of thermodynamic parameters. SAXS didn’t show of melafen influence at wide range of concentrations on the bilayers thickness and packing at natural multilamellar phospholipid liposomes.

It has been concluded that the approach based on the using of these experimental methods and objects could be applied to the substances test with the aim of revealing of their biological activity.


1. Chen W., Kudryashev M. Structure of RyR1 in native membrane. // Second Russian international conference “Cryo-electron microscopy. 2019: achievements and prospects” Lomonosov Moscow State University (MSU) June 2 – 5, 2019 pp. 36-37.

2.Archipova G.V., Burlakova E.B., Krivandin A.V., Pogoretskaya I.L. Phenosan-acid influence to phospholipid membrane. // Neurochemistry volume 13, 1996. P. 128-132 (in Russian).

3. Krivandin A.V., Fatkullina L.D., Shatalova O.V., Goloshchapov A.N., Burlakova E.B. Investigation of antioxidant IHFAN incorporation to liposomes by method small angle Rentgen scattering. Chemical Physics. volume 32, 2013. P. 91-96. (in Russian).

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